Growth requirements of pyruvate-decarboxylase-negative Saccharomyces cerevisiae
β Scribed by Marcel T. Flikweert; Martin de Swaaf; Johannes P. van Dijken; Jack T. Pronk
- Book ID
- 117519201
- Publisher
- John Wiley and Sons
- Year
- 1999
- Tongue
- English
- Weight
- 203 KB
- Volume
- 174
- Category
- Article
- ISSN
- 0378-1097
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In Sacchuromyces cerevisiue, the structural genes PDCI, PDCS and PDC6 each encode an active pyruvate decarboxylase. Replacement mutations in these genes were introduced in a homothallic wild-type strain, using the dominant marker genes APT1 and TnSble. A pyruvate-decarboxylase-negative (Pdc -) mutan
## Abstract Oxythiamin is one of the antivitamin derivatives of thiamin which, after phosphorylation, can be bound to the catalytic centre of thiaminβdependent enzymes and inhibit these enzymes. In this work the influence of oxythiamin on the growth rate, survival and the activity of pyruvate decar