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An internal deletion inMTH1enables growth on glucose of pyruvate-decarboxylase negative, non-fermentativeSaccharomyces cerevisiae

✍ Scribed by Oud, Bart; Flores, Carmen-Lisset; Gancedo, Carlos; Zhang, Xiuying; Trueheart, Joshua; Daran, Jean-Marc; Pronk, Jack T; van Maris, Antonius JA

Book ID: 119900483
Publisher: BioMed Central
Year: 2012
Tongue: English
Weight: 334 KB
Volume: 11
Category: Article
ISSN: 1475-2859
DOI: 10.1186/1475-2859-11-131

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In Sacchuromyces cerevisiue, the structural genes PDCI, PDCS and PDC6 each encode an active pyruvate decarboxylase. Replacement mutations in these genes were introduced in a homothallic wild-type strain, using the dominant marker genes APT1 and TnSble. A pyruvate-decarboxylase-negative (Pdc -) mutan