✦ LIBER ✦

Growth requirements of human mammary epithelial cells in culture

✍ Scribed by J. Taylor-Papadimitriou; M. Shearer; M. G. P. Stoker

Publisher: John Wiley and Sons
Year: 1977
Tongue: French
Weight: 553 KB
Volume: 20
Category: Article
ISSN: 0020-7136
DOI: 10.1002/ijc.2910200613

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Colony‐forming epithelial cells can be separated from the non‐dividing “foam cells” in human milk by differential adhesion to glass and freezing. The growth of such partially purified mammary epithelial cells is stimulated by co‐culture with non‐dividing feeder cells. Foam cells, mitomycin‐treated mouse fibroblast lines and human mammary fibroblasts and calf lens epithelial cells are all effective in promoting mammary epithelial cell growth. Contact between epithelial cells and feeders is not required for the growth‐promoting effect. The mitogenic effect of epidermal growth factor on mammary epithelial cells also requires feeder cell activity.

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