Serum-free medium for murine and human l
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Masatoshi Togami; Kenji Yasuda; Masao Kariya
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Article
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1991
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Springer
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English
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We established a serum-free medium of low protein content (125 micrograms/ml) TYI 100, consisting of three hormones and five growth factors for the growth of lymphoid and hybridoma cell lines. In TYI 100 medium, mouse and human hybridomas grew equally well as in RPMI 1640 supplemented with 10% fetal