Growth of human B-lymphocyte colonies in vitro
β Scribed by RADNAY, J.; GOLDMAN, I.; ROZENSZAJN, L. A.
- Book ID
- 109713473
- Publisher
- Nature Publishing Group
- Year
- 1979
- Tongue
- English
- Weight
- 686 KB
- Volume
- 278
- Category
- Article
- ISSN
- 0028-0836
- DOI
- 10.1038/278351a0
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## Abstract Bβlymphocyte colonies were grown in semiβsolid agar from mouse spleen or lymph node cells in the presence of mercaptoethanol with or without added sheep red cells. High levels of colonyβforming cells were present in the spleen or normal mice and nu/nu (athymic) mice but colonyβforming c
## Abstract Murine lymphoid cells which were stimulated in liquid culture containing thymus culture fluid (ThyβCF) and seeded in a soft agar culture system, proliferated and developed into B cell colonies. Two types of colonies were formed: large colonies within the upper layer and small flat colon