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Growth inhibition of human colorectal-carcinoma cells by interleukin-4 and expression of functional interleukin-4 receptors

✍ Scribed by Harald Lahm; Bruno Schnyder; Josiane Wyniger; Zita Borbenyi; Aysim Yilmaz; Bruce D. Car; Jürgen R. Fischer; Jean-Claude Givel; Bernhard Ryffel

Publisher
John Wiley and Sons
Year
1994
Tongue
French
Weight
875 KB
Volume
59
Category
Article
ISSN
0020-7136

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✦ Synopsis

Abstract

The growth‐inhibitory effect of interleukin‐4 (IL‐4) was investigated in a panel of 7 human colorectal‐carcinoma cell lines. In 5 cell lines (HT29, WiDr, LS41 IN, LS513, LS1034) a dosedependent reduction of proliferation was documented. At 100 U/ml, IL‐4 inhibited thymidine incorporation between 45 and 75% and MTT conversion (26 to 41%). The ability of LS513 and WiDr cells to form colonies after IL‐4 treatment was reduced by 85 and 62% respectively. LS513 was the most sensitive cell line, with IL‐4 inducing half‐maximal inhibition at 5 to 6 U/ml. The inhibitory effect of IL‐4 was completely neutralized by anti‐IL‐4 antibodies. Northern‐blot analysis revealed the presence of IL‐4‐receptor (IL‐4R) mRNA in all cell lines. The membrane expression of the 130‐kDa IL‐4R was assessed by FACS, utilizing an anti‐IL‐4R monoclonal antibody and was confirmed by biotinylated IL‐4 binding. Our results attribute an important role for IL‐4 as a negative regulator of colorectal‐carcinoma cell growth, thus indicating a possible avenue for intervention in this disease. © 1994 Wiley‐Liss, Inc.

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