Growth dynamics of mammalian cells monitored with automated cell cycle staining and flow cytometry
โ Scribed by Greg Sitton; Friedrich Srienc
- Publisher
- John Wiley and Sons
- Year
- 2008
- Tongue
- English
- Weight
- 660 KB
- Volume
- 73A
- Category
- Article
- ISSN
- 0196-4763
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Flow cytometry was used to measure cell cycle parameters in Solanum aviculare plant cell suspensions. Methods for bromodeoxyuridine (BrdU) labeling of plant nuclei were developed so that cell cycle times and the proportion of cells participating in growth could be determined as a function of culture
This study compares the cell cycle distribution in rat thymocytes obtained by means of bromodeoxyuridine (BrdUrd) labeling of S-phase cells and the analysis of the S-phase fraction obtained according t o the technique ofvindelov et al. (Cytometry 3332-338,1983). The proportion of BrdUrd-labeled cell