✦ LIBER ✦

Growth control of normal and transformed cells

✍ Scribed by Riddle, Veronica G. H. ;Pardee, Arthur B. ;Rossow, Peter W.

Publisher: Wiley (John Wiley & Sons)
Year: 1979
Tongue: English
Weight: 554 KB
Volume: 11
Category: Article
ISSN: 0091-7419
DOI: 10.1002/jss.400110411

No coin nor oath required. For personal study only.

✦ Synopsis

Both serum factors and protein synthesis are required for normal cell growth. Swiss 3T3 cells require the serum growth factors insulin and EGF (epidermal growth factor) during the initial part of the G I period, until they pass a restriction point about 2 h before the initiation of DNA synthesis. Concentrations of cycloheximide that inhibit protein synthesis by as much as 70% dramatically lengthen the cell cycle before the restriction point, while the cell cycle after the restriction point remains nearly constant. These results are consistent with a model in which labile proteins are required for transit of cells past the serum-sensitive restriction point. The relation of these findings t o the growth control of transformed cells is discussed.

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