Enucleation of normal and transformed cells

✍ Scribed by Douglas Degaetano; Melvin Schindler

• Publisher: John Wiley and Sons
• Year: 1987
• Tongue: English
• Weight: 1003 KB
• Volume: 130
• Category: Article
• ISSN: 0021-9541
• DOI: 10.1002/jcp.1041300217

✦ Synopsis

A quantitative analysis based on centrifugal force requirements for enucleation was developed to examine the response of a number of untransformed and transformed cell lines to cytochalasin mediated enucleation. Examination of the extent of cell enucleation as a function of centrifugal force resulted in a series of response curves demonstrating that enucleation g force requirements varied between Balblc 3T3, Swiss 3T3, and Kirsten sarcoma virus transformed Balblc 3T3 (3T3-K). A four times greater centrifugal force was required to reach 50% enucleation for transformed Balblc 3T3-K when compared to Swiss 3T3. A qualitative correlation could be observed between ease of enucleation and the existence of a well-formed stress fiber network. A comparison of cytochalasin B and D suggested that cytochalasin D was far more effective in the enucleation of transformed cells. Experiments with 2-deoxyglucose and monensin provided evidence that decreasing cellular ATP levels, either directly or potentially by uncoupling ion transport from ATP generation, can decrease the efficiency of enucleation. It is suggested that the organization of the cytoskeleton is affected by the altered cellular ATP levels which can affect the centrifugal requirements of enucleation.