Growth characteristics of Escherichia coliHB101[pGEc47] on defined medium

✍ Scribed by S. A. Rothen; M. Sauer; B. Sonnleitner; B. Witholt

• Book ID: 101240662
• Publisher: John Wiley and Sons
• Year: 1998
• Tongue: English
• Weight: 133 KB
• Volume: 58
• Category: Article
• ISSN: 0006-3592
• DOI: 10.1002/(sici)1097-0290(19980405)58:1<92::aid-bit10>3.0.co;2-7

✦ Synopsis

This paper shows that differences in growth behavior of Escherichia coli strain HB101 and strain HB101[pGEc47] can be related to yeast extract-enriched medium rather than plasmid properties. An optimal medium for growth of E. coli HB101[pGEc47] was designed based on the individual yield coefficients for specific medium components (NH 4 + 6 g g -1 , PO 4 3-14 g g -1 , SO 4 2-50 g g -1 ). The yield coefficient for L-leucine depends on the glucose content of the medium (20 g g -1 for 3% glucose, 40 g g -1 for 1% glucose) and the yield coefficient for L-proline depends on the cultivation mode (20 g g -1 for batch cultivation, 44 g g -1 for continuous cultivation). Growth on defined medium after medium optimization is as rapid as on complex medium (0.42-0.45 h -1 ). The critical dilution rate (D R ) in the defined medium above which undesired production of acetic acid occurs is in the range of 0.23-0.26 h -1 .