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Growth and metabolism of hybridomas immobilized in packed beds: comparison with static and suspension cultures

✍ Scribed by A.D. Murdin; J.S. Thorpe; D.J. Groves; R.E. Spier

Publisher: Elsevier Science
Year: 1989
Tongue: English
Weight: 447 KB
Volume: 11
Category: Article
ISSN: 0141-0229
DOI: 10.1016/0141-0229(89)90017-3

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## Abstract The steady‐state metabolic parameters for a hybridoma cell line have been determined in continuous suspension–perfusion culture over a wide range of perfusion rates and cell bleed rates. Significant increases in viable cell concentrations and volumetric productivities were achieved at h