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Growth and Hepatospecific Gene Expression of Human Hepatoma Cells in a Defined Medium

โœ Scribed by G. J. Darlington, J. H. Kelly and G. J. Buffone


Book ID
125620518
Publisher
Society for In Vitro Biology
Year
1987
Tongue
English
Weight
286 KB
Volume
23
Category
Article
ISSN
1475-2689

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A choline non-required cell line was established from a rat hepatoma cell line. The line designated R-YI21B.cho was able to grow in choline-deprived medium without serum and lipid. Choline is necessary for the biosynthesis of phosphatidylcholine, which is a main component of cell membranes. Phosphat