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Growth and hepatospecific gene expression of human hepatoma cells in a defined medium

โœ Scribed by G. J. Darlington; J. H. Kelly; G. J. Buffone

Book ID
112799421
Publisher
Society for In Vitro Biology
Year
1987
Tongue
English
Weight
428 KB
Volume
23
Category
Article
ISSN
1475-2689

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A choline non-required cell line was established from a rat hepatoma cell line. The line designated R-YI21B.cho was able to grow in choline-deprived medium without serum and lipid. Choline is necessary for the biosynthesis of phosphatidylcholine, which is a main component of cell membranes. Phosphat