Growth and Development of Mouse Oocytes in Vitro
β Scribed by JOHN J. EPPIG; SUSAN A. J. DANIEL; ALLEN C. SCHROEDER
- Book ID
- 119860891
- Publisher
- John Wiley and Sons
- Year
- 1988
- Tongue
- English
- Weight
- 319 KB
- Volume
- 541
- Category
- Article
- ISSN
- 0890-6564
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## Abstract A method is described for in vitro culture of naked growing mouse oocytes for at least four days. Tests for metabolic function indicate that the oocytes increase in volume, accumulate glucoseβ6βphosphate dehydrogenase, and maintain a steady level of ^3^Hβuridine and ^3^Hβleucine incorpo
Oocytes isolated from mature follicles undergo spontaneous maturation when cultured in vitro. Forskolin, an adenylate cyclase stimulator, inhibited resumption of meiosis of cumulus-free mouse oocytes in vitro. Germinal vesicle breakdown (GVBD) was prevented in more than 85% of the oocytes treated by