## Abstract Thousands of proteins may be visualised on a twoβdimensional (2βD) gel, but only hundreds are present at levels sufficient for chemical analysis. Therefore, prefractionation of protein samples prior to 2βD polyacrylamide gel electrophoresis (PAGE) will be important for the investigation
Gradiflow as a prefractionation tool for two-dimensional electrophoresis
β Scribed by Vicki L. Locke; Toby S. Gibson; Theresa M. Thomas; Garry L. Corthals; Dennis B. Rylatt
- Book ID
- 101389466
- Publisher
- John Wiley and Sons
- Year
- 2002
- Tongue
- English
- Weight
- 225 KB
- Volume
- 2
- Category
- Article
- ISSN
- 1615-9853
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
## Abstract Despite its excellent resolving power, 2βDE is of limited use when analyzing cellular proteomes, especially in differential expression studies. Frequently, fewer than 2000 protein spots are detected on a single 2βD gel (a fraction of the total proteome) regardless of the gel platform, s
## Two-dimensional electrophoresis protein profiling as an analytical tool for human acute leukemia classification Two-dimensional electrophoresis (2-DE) was used to profile the proteins of leukemic cells from 61 cases of akute leukemia (AL) characterized by the French-American-British (FAB) classi