Gradient elution in hollow-fibre flow field-flow fractionation

✍ Scribed by Alf Carlshaf; Jan Åke Jönsson

• Book ID: 104144619
• Publisher: Elsevier Science
• Year: 1989
• Tongue: English
• Weight: 318 KB
• Volume: 461
• Category: Article
• ISSN: 1873-3778
• DOI: 10.1016/s0021-9673(00)94277-8

✦ Synopsis

The scope of the technically simple hollow-fibre version fo field-flow fractionation has been extended to gradient elution. An arrangement of two liquid chromatographic pumps and a gradient controller permits sample introduction, relaxation and elution under gradient conditions to be conveniently performed. The retention times experimentally found can be theoretically explained. The technique was applied to model separations of polystyrene latex beads and plasmids.