Gradient and isocratic high-performance hydrophobic interaction chromatography of proteins on agarose columns
✍ Scribed by Stellan Hjertén; Kunquan Yao; Kjell-ove Eriksson; Bodil Johansson
- Publisher
- Elsevier Science
- Year
- 1986
- Tongue
- English
- Weight
- 692 KB
- Volume
- 359
- Category
- Article
- ISSN
- 1873-3778
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## Abstract A microchromatographic procedure for highly sensitive analysis of proteins has been developed by using short capillary columns with reverse‐phase, ion‐exchange, and hydrophobic interaction sorbents. The high analytical mass sensitivity on the level of several nanograms was attained by t
Curves showing the relation between log (molecular weight) and distribution coefficient are presented for proteins subjected to molecular sieve chromatography on crosslinked and non-crosslinked agarose gels of different concentrations. These curves, which facilitate selection of the gel concentratio