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Glycogen synthase from human and bovine polymorphonuclear leukocyte Immunochemical characterization and comparison to glycogen synthase from rat and rabbit muscle and liver cells

✍ Scribed by Lars Hvilsted Rasmussen; Henning Juhl; Viggo Esmann; Hans Uffe Petersen

Book ID
115920783
Publisher
Elsevier Science
Year
1988
Tongue
English
Weight
610 KB
Volume
230
Category
Article
ISSN
0014-5793

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Glycogen synthase kinase-3 (GSK-3) was purified from rabbit liver to homogeneity by ultracentrifugation, ion-exchange chromatography on DEAE-cellulose, Cellulose phosphate, CM-Sephadex and Fast Protein Liquid Chromatography (FPLC) on Mono-S column. The enzyme was purified approximately 20,000 fold w