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Purification and characterization of two cyclic AMP-independent casein/glycogen synthase kinases from rat liver cytosol

✍ Scribed by Emilio Itarte; M. Angels Mor; Agustí Salavert; J. Manuel Pena; JoséFernando Bertomeu; Joan J. Guinovart


Book ID
113124442
Publisher
Elsevier Science
Year
1981
Weight
746 KB
Volume
658
Category
Article
ISSN
0005-2744

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✍ Rabindra Randhawa; Ramji L. Khandelwal 📂 Article 📅 1990 🏛 Springer 🌐 English ⚖ 721 KB

Glycogen synthase kinase-3 (GSK-3) was purified from rabbit liver to homogeneity by ultracentrifugation, ion-exchange chromatography on DEAE-cellulose, Cellulose phosphate, CM-Sephadex and Fast Protein Liquid Chromatography (FPLC) on Mono-S column. The enzyme was purified approximately 20,000 fold w