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Glycerol acyl-transfer kinetics of a circular permutated Candida antarctica lipase B

✍ Scribed by Joseph A. Laszlo; Ying Yu; Stefan Lutz; David L. Compton


Book ID
113804875
Publisher
Elsevier Science
Year
2011
Tongue
English
Weight
487 KB
Volume
72
Category
Article
ISSN
1381-1177

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## Abstract The engineering of lipase B from __Candida antarctica__ (CALB) by circular permutation has yielded over sixty hydrolase variants, and several show significantly improved catalytic performance. Here we report a detailed characterization of ten selected enzyme variants by kinetic and spec