Several methods are available for the extraction and quantitation of oxidized and reduced pyridine nucleotides in erythrocytes. Enzymatic methods, however, are complicated by the presence of hemoglobin, which causes oxidation of NADH and NADPH during extraction. Although hemoglobin-mediated oxidatio
Glutathione: A source of interference in pyridine nucleotide determinations by a cycling procedure☆
✍ Scribed by BERNOFSKY, C; ROYAL, K
- Book ID
- 121771040
- Publisher
- Elsevier Science
- Year
- 1970
- Tongue
- English
- Weight
- 257 KB
- Volume
- 215
- Category
- Article
- ISSN
- 0304-4165
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In the course of an investigation of the properties of 2,2'-dithiodipyri-dine2 and its effects on animal cells, we found that this compound oxidizes thiols rapidly to the corresponding disulfide (1)) and that this reaction can be coupled to metabolic reactions, such as the reduction of GSSG by TPNH
Quantitative analysis of red cell pyridine nucleotides has been unreliable in the past because of technical problems in extracting them in the presence of hemoglobin. A simple alcoholic extraction procedure for analysis of pyridine nucleotides in red blood cells is described in this paper. Pyridine