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The use of 2,2′-dithiodipyridine in the determination of glutathione and of triphosphopyridine nucleotide by enzymatic cycling

✍ Scribed by D.R. Grassetti; J.F. Murray Jr.

Publisher
Elsevier Science
Year
1967
Tongue
English
Weight
361 KB
Volume
21
Category
Article
ISSN
0003-2697

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✦ Synopsis

In the course of an investigation of the properties of 2,2'-dithiodipyri-dine2 and its effects on animal cells, we found that this compound oxidizes thiols rapidly to the corresponding disulfide (1)) and that this reaction can be coupled to metabolic reactions, such as the reduction of GSSG by TPNH (2). When 2-PDS reacts with a thiol the products are a disulfide and 2-TP:

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