Glutamine synthetase gene expression and glutamate transporters in C6-glioma cells
β Scribed by Zafeer Baber; Nasrin Haghighat
- Publisher
- Springer
- Year
- 2010
- Tongue
- English
- Weight
- 315 KB
- Volume
- 25
- Category
- Article
- ISSN
- 0885-7490
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π SIMILAR VOLUMES
Previous studies from this laboratory have shown that C-6 rat glioma cells (2B clone) exhibit specific phenotypic characteristics depending on passage in culture and that these populations respond differentially to addition of various exogenous compounds to the medium. Early passage (<25) C-6 glial
## Abstract A previous study has shown that glutamine (Gln) uptake in C6 cells grown in a standard medium containing 2 mM Gln, is predominantly mediated by a sodiumβdependent system that is inhibited by ASC system substrates alanine (Ala), serine (Ser), cysteine (Cys) and threonine (Thr), shows pH
Metabolite control of glutamine synthetase expression (by glutamine) was studied in L6 skeletal muscle cells. Depletion of glutamine from the culture medium for 24 hours resulted in a 3-4-fold increase in glutamine synthetase activity. This effect was blocked by cycloheximide but not by actinomycin
## Abstract In brain, levels of adenosine increase up to 100βfold during cerebral ischemia. Based on in vitro studies, both astrocytes and neurons contribute to this adenosine release. Neurons release adenosine per se whereas astrocytes release adenine nucleotides that are metabolized to adenosine