Glutamate transport in cultures from developing avian cerebellum: Presence of GLT-1 immunoreactivity in Purkinje neurons

✍ Scribed by Jennifer A. Meaney; Vladimir J. Balcar; Jeffrey D. Rothstein; Peter L. Jeffrey

• Publisher: John Wiley and Sons
• Year: 1998
• Tongue: English
• Weight: 349 KB
• Volume: 54
• Category: Article
• ISSN: 0360-4012
• DOI: 10.1002/(sici)1097-4547(19981201)54:5<595::aid-jnr4>3.0.co;2-q

✦ Synopsis

Immunocytochemical studies indicated that Purkinje cells cultured from chick embryonic cerebellum (embryonic day 8) strongly express a glutamate transporter EAAT2 cloned from human brain (GLT-1 in rat brain). At both 7 days and 14 days in culture, Purkinje neurons accumulated 1 M [ 3 H]L-glutamate via a potent ''high-affinity'' transport system that could be inhibited by D-and L-threo-3-hydroxyaspartate (D-and L-t-3OHA) and by L-trans-pyrrolidine-2,4dicarboxylate (L-t-PDC). The order of potency of the three inhibitors was L-t-PDC D L-t-3OHA G D-t-3OHA. Only the value of IC 50 (concentration causing 50% inhibition) for D-t-3OHA significantly changed between 7 days (116 M) and 14 days in culture (40 M). All n H D 1, except in the case of the inhibition by D-t-3OHA at 14 days in culture (n H ‫؍‬ 0.57), indicating the possible appearance of heterogeneity of the transport sites at later stages of culturing. Chronic inhibition of L-glutamate transport by L-t-PDC resulted in major changes in the morphology of Purkinje cells; particularly, the neurites almost completely regressed.