Glucose Transport: Methods and Protocols

✍ Scribed by Karin Lindkvist-Petersson,Jesper S Hansen (eds.)

• Publisher: Humana Press
• Year: 2018
• Tongue: English
• Leaves: 270
• Series: Methods in Molecular Biology 1713
• Edition: 1
• Category: Library

✦ Synopsis

This volume explores methods used to examine the structure-function relationship of glucose transporters both in vitro and ex vivo. Chapters in this book cover topics such as expression and purification of glucose transporters in heterologous expression systems, crystal structure determination, glucose transport activity assays in cellular and synthetic model systems, investigation of the GLUT4 membrane trafficking machinery by novel labeling strategies combined with advanced fluorescence microscopy methods, and tracking GLUT2 translocation by live cell imaging. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, Glucose Transport: Methods and Protocols is a valuable resource that helps researchers with determining specific roles of different GLUTs in various organisms.

✦ Table of Contents

Front Matter ....Pages i-xi
Expression and Purification of Rat Glucose Transporter 1 in Pichia pastoris (Raminta Venskutonytė, Karin Elbing, Karin Lindkvist-Petersson)....Pages 1-13
Crystallization and Structural Determination of the Human Glucose Transporters GLUT1 and GLUT3 (Dong Deng, Nieng Yan)....Pages 15-29
Screening and Scale-Up of GLUT Transporter Constructs Suitable for Biochemical and Structural Studies (Grégory Verdon, Hae Joo Kang, David Drew)....Pages 31-43
GLUT Characterization Using Frog Xenopus laevis Oocytes (Wentong Long, Debbie O’Neill, Chris I. Cheeseman)....Pages 45-55
Glucose Uptake in Heterologous Expression Systems (Eunice E. Lee, Richard C. Wang)....Pages 57-67
Evaluating the Efficacy of GLUT Inhibitors Using a Seahorse Extracellular Flux Analyzer (Changyong Wei, Monique Heitmeier, Paul W. Hruz, Mala Shanmugam)....Pages 69-75
Glucose Transport Activity Measured in Giant Vesicles (Jesper S. Hansen, Karin Lindkvist-Petersson)....Pages 77-91
Design, Synthesis, and Evaluation of GLUT Inhibitors (Carlotta Granchi, Tiziano Tuccinardi, Filippo Minutolo)....Pages 93-108
Applying Microfluidic Systems to Study Effects of Glucose at Single-Cell Level (Niek Welkenhuysen, Caroline B. Adiels, Mattias Goksör, Stefan Hohmann)....Pages 109-121
A Growth-Based Screening System for Hexose Transporters in Yeast (Eckhard Boles, Mislav Oreb)....Pages 123-135
Identification of Insulin-Activated Rab Proteins in Adipose Cells Using Bio-ATB-GTP Photolabeling Technique (Francoise Koumanov, Geoffrey D. Holman)....Pages 137-150
Total Internal Reflection Fluorescence Microscopy to Study GLUT4 Trafficking (Sebastian Wasserstrom, Björn Morén, Karin G. Stenkula)....Pages 151-159
Translocation and Redistribution of GLUT4 Using a Dual-Labeled Reporter Assay (Robert M. Jackson, Ann Louise Olson)....Pages 161-173
GLUT4 Translocation in Single Muscle Cells in Culture: Epitope Detection by Immunofluorescence (Javier R. Jaldin-Fincati, Philip J. Bilan, Amira Klip)....Pages 175-192
Glucose Transport: Methods for Interrogating GLUT4 Trafficking in Adipocytes (Dougall M. Norris, Tom A. Geddes, David E. James, Daniel J. Fazakerley, James G. Burchfield)....Pages 193-215
Proximity Ligation Assay to Study the GLUT4 Membrane Trafficking Machinery (Dimitrios Kioumourtzoglou, Gwyn W. Gould, Nia J. Bryant)....Pages 217-227
Quantification of Cell-Surface Glucose Transporters in the Heart Using a Biotinylated Photolabeling Assay (Zahra Maria, Véronique A. Lacombe)....Pages 229-240
Tracking GLUT2 Translocation by Live-Cell Imaging (Sabina Tsytkin-Kirschenzweig, Merav Cohen, Yaakov Nahmias)....Pages 241-254
GLUT2-Expressing Neurons as Glucose Sensors in the Brain: Electrophysiological Analysis (Gwenaël Labouèbe, Bernard Thorens, Christophe Lamy)....Pages 255-267
Back Matter ....Pages 269-274

✦ Subjects

Cell Biology