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Glycomics: Methods and Protocols

✍ Scribed by Willy Morelle, Valegh Faid, Frédéric Chirat, Jean-Claude Michalski (auth.), Niclas G. Karlsson, Nicolle H. Packer (eds.)


Publisher
Humana Press
Year
2009
Tongue
English
Leaves
379
Series
Methods in Molecular Biology 534
Edition
1
Category
Library

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✦ Synopsis


Due to the significant contributions of carbohydrates to the functional diversity of the cell, the challenging study of the glycome has expanded beyond the research of carbohydrate experts and into the wider scope of the life sciences. To aid all scientists now delving into this vital subject area, Glycomics: Methods and Protocols collects a compendium of detailed laboratory protocols reflecting the increasing availability of sample preparation, chromatographic, electrophoretic, mass spectrometric, and bioinformatic tools specifically designed for the analysis of glycosylation. Leading researchers in the field address subjects such as glycoprotein and proteoglycan analysis, glycosylation structure determination, as well as various approaches to investigate the interaction between glycans and a variety of carbohydrate-recognizing proteins in order to aid exploration into the functional significance of the oligosaccharides. Written in the highly successful Methods in Molecular Biologyβ„’ series format, the chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Glycomics: Methods and Protocols serves as a valuable guide for experimenters facing the challenges of glycan analysis in hope of providing further insights into the biology of cell-cell communication and interaction.

✦ Table of Contents


Front Matter....Pages ii-xiv
Front Matter....Pages 1-2
Front Matter....Pages 3-21
Front Matter....Pages 23-35
Front Matter....Pages 37-51
Front Matter....Pages 53-64
Front Matter....Pages 65-77
Back Matter....Pages 79-91
....Pages 93-105

✦ Subjects


Biochemistry, general; Human Physiology; Carbohydrate Chemistry


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