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Glucose transport in a methylotrophic yeast Hansenula polymorpha

✍ Scribed by Helen Karp; Tiina Alamäe

Book ID
109322602
Publisher
John Wiley and Sons
Year
1998
Tongue
English
Weight
631 KB
Volume
166
Category
Article
ISSN
0378-1097

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We developed a host-vector system for transformation and gene cloning experiments using the methylotrophic yeast Hansenula p o l y w p h a . Regeneration of protoplasts in a medium containing polyethylene glycol before plating made transformation more efficient and reproducible (2 to 3 -lo4 pg DNA).