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Glucose transport and metabolism in cultured cells of nervous tissue

✍ Scribed by Janet V. Passonneau

Publisher
John Wiley and Sons
Year
1976
Tongue
English
Weight
291 KB
Volume
89
Category
Article
ISSN
0021-9541

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✦ Synopsis

Abstract

The effect of the concentration of glucose in the medium on the intracellular concentrations of metabolites in C‐6 astrocytoma cells and C‐1300 neuroblastoma cells in culture has been investigated. The intracellular concentrations of glucose, glycogen, glucose 6‐P and UDP‐glucose were measured at intervals after feeding the cells. A rapid increase in glucose and glucose 6‐P levels occurred when fresh medium containing 5.5 mM glucose was applied to the cells, followed by slower increases in UDP‐glucose and glycogen. When the medium glucose was increased ten‐fold, the intracellular concentration of glucose was increased, but the levels of glucose 6‐P, UDP‐glucose and glycogen were not altered, nor were the rates of accumulation. The addition of insulin to the medium resulted in an increase of intracellular glucose, glucose 6‐P and glycogen. The transport of glucose into the cells is not the rate‐limiting step of the regulation of metabolite levels in the cells.

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