GLUT1-mediated glucose transport and its regulation by IGF-I in cultured bovine chromaffin cells
✍ Scribed by Catherine Fladeby; Birgitte Bjønness; Guldborg Serck-Hanssen
- Publisher
- John Wiley and Sons
- Year
- 1996
- Tongue
- English
- Weight
- 727 KB
- Volume
- 169
- Category
- Article
- ISSN
- 0021-9541
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✦ Synopsis
Regulation of glucose transport was studied in primary cultures of bovine chromaffin cells (BCC) using the glucose analogue 2-deoxyglucose (DOG) as a model substrate. The glucose transporter in freshly isolated and cultured BCC was identified as GLUT1 by Western immunoblots. The level of GLUTl increased by time in culture and was followed by an enhancement in uptake of DOG. The DOG uptake was stimulated by insulin-like growth factor I (IGF-I) with an EC,,, of 1 nM and a maximal response (-2-fold) was obtained at 10-1 00 nM IGF-I. Insulin was at least 100-fold less potent than IGF-I. Exposure to 1 0-8 M IGF-I also caused a redistribution of GLUT1 from an intracellular compartment to a plasma membrane-enriched fraction. Our results demonstrate a GLUT1 -mediated glucose uptake in adrenomedullary cells. An enhanced glucose transport in response to IGF-I appears to be coupled to activation of ICF receptor type 1 and GLUT1 translocation. 0 1996 Wiley-Liss, Inc