Glucose metabolism of isolated mammalian Islets of Langerhans; effects of glucagon, 2-deoxy-D-glucose and D-mannoheptulose
β Scribed by R. J. Jarrett; H. Keen
- Book ID
- 105151487
- Publisher
- Springer
- Year
- 1968
- Tongue
- English
- Weight
- 442 KB
- Volume
- 4
- Category
- Article
- ISSN
- 0012-186X
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In pancreatic islets prepared from fed rats and incubated at a low concentration (1 . 7 mM) of D-glucose, D-mannoheptulose (10 . 0 mM) virtually fails to aect the metabolism of the hexose. Likewise, in islets from starved rats, the relative extent of the inhibitory action of D-mannoheptulose upon D-
A modification of Keston's glucose oxidase method for D-glucose analysis is described. The glucose oxidase-peroxidase reaction was carried out in phosphate buffer, pH 5.9 at 45 Β°, for 30 minutes. A clear stable color was developed by addition of sulfuric acid to a final concentration of 30 per cent.