Glucose enzyme electrode with extended linearity: Application to undiluted blood measurements

✍ Scribed by William H. Mullen; Fiona H. Keedy; Stephen J. Churchouse; Pankaj M. Vadgama

• Publisher: Elsevier Science
• Year: 1986
• Tongue: English
• Weight: 522 KB
• Volume: 183
• Category: Article
• ISSN: 0003-2670
• DOI: 10.1016/0003-2670(86)80074-5

✦ Synopsis

An enzyme electrode is described for glucose determination in unstirred, undiluted whole blood. The system comprises an H,O,-detecting electrode upon which is placed a membrane laminate incorporating glucose oxidase. The external membrane was pretreated with methyltrichlorosilane.

The electrode response was linearly dependent on glucose concentration up to 50 mmol I-' glucose, it had a decreased dependence on dissolved oxygen concentrations and gave response times of 30-90 s. Whole blood glucose measurements correlated well with a routine spectrophotometric method.