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Glucose-based optimization of CHO-cell perfusion cultures

✍ Scribed by Jason E. Dowd; K. Ezra Kwok; James M. Piret


Book ID
101720414
Publisher
John Wiley and Sons
Year
2001
Tongue
English
Weight
140 KB
Volume
75
Category
Article
ISSN
0006-3592

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## Abstract To investigate the effect of culture temperature on erythropoietin (EPO) production and glycosylation in recombinant Chinese hamster ovary (CHO) cells, we cultivated CHO cells using a perfusion bioreactor. Cells were cultivated at 37Β°C until viable cell concentration reached 1 × 10^7^ c