Improvement of CHO Cell Culture Medium Formulation: Simultaneous Substitution of Glucose and Glutamine
✍ Scribed by C. Altamirano; C. Paredes; J. J. Cairó; F. Gòdia
- Book ID
- 109388312
- Publisher
- American Institute of Chemical Engineers
- Year
- 2000
- Tongue
- English
- Weight
- 109 KB
- Volume
- 16
- Category
- Article
- ISSN
- 8756-7938
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Rates of CO2 production from glucose and glutamine, intracellular metabolite levels, and release of metabolic end products into the culture medium were determined for 13 cultured cell lines, including a glycolysis-defective mutant. All the non-mutant lines synthesized pyruvate, lactate, alanine, pro