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Glucose-6-phosphate as a probe for the glucosamine-6-phosphate N-acetyltransferase Michaelis complex

โœ Scribed by Ramon Hurtado-Guerrero; Olawale Raimi; Sharon Shepherd; Daan M.F. van Aalten


Book ID
118442808
Publisher
Elsevier Science
Year
2007
Tongue
English
Weight
215 KB
Volume
581
Category
Article
ISSN
0014-5793

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A method for the determination of glucos
โœ S.A. Kitcher; K. Siddle; J.P. Luzio ๐Ÿ“‚ Article ๐Ÿ“… 1978 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 414 KB

A method is described for measuring the activity of glucose-6-phosphatase (EC 3.1.3.9) in rat liver. [U-W]Glucose 6-phosphate, as substrate, is converted by the enzyme to [Ylglucose and inorganic phosphate. The addition of ZnSO, and Ba(OH), at the end of the reaction precipitates phosphate and the u