Glucose-6-phosphatase activity in rat liver parenchyma during azo-dye carctnogenesis

✍ Scribed by Marie-Christine Moulin; Roger Daoust

• Publisher: John Wiley and Sons
• Year: 1971
• Tongue: French
• Weight: 569 KB
• Volume: 8
• Category: Article
• ISSN: 0020-7136
• DOI: 10.1002/ijc.2910080111

✦ Synopsis

Abstract

The glucose‐6‐phosphatase (C‐6‐Pase) activity was investigated by the Wachstein and Meisel method in preneoplastic rat liver parenchyma, as well as in normal liver and hepatomas induced by 4‐dimethylaminoazobenzene (DAB).

Normal liver shows a high G‐6‐Pase activity restricted to the cytoplasm of hepatocytes. At early stages of DAB feeding, the centrolobular degenerating zones give a coarser reaction while portal areas retain a normal activity. In cirrhotic liver, the intensity of the reaction varies greatly from one nodule of regeneration to another but is relatively uniform within each nodule.

Hyperbasophilic foci are found in nodules with either high or low G‐6‐Pase activity. The foci developing from highly active nodules show a loss of G‐6‐Pase activity and the tumors are negative.

It appears from these studies that a) the variations in G‐6‐Pase activity observed in regenerating nodules play no role in the development of hyperbasophilic foci and hepatomas, and b) the loss of activity occurring in hyperbasophilic foci and tumors probably represents a secondary feature of the neoplastic transformation.