Gluconeogenesis in hepatocytes determined with [2-13C] acetate and quantitative 13C NMR spectroscopy
β Scribed by Kitt Falk Petersen; Niels Grunnet
- Publisher
- Elsevier Science
- Year
- 1993
- Tongue
- English
- Weight
- 581 KB
- Volume
- 25
- Category
- Article
- ISSN
- 0020-711X
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The relationship between the phosphoenergetic state and gluconeogenesis in the liver after ischemic damage was investigated using living rats. The ATP level was determined with in vivo 31 P nuclear magnetic resonance spectroscopy, and gluconeogenesis was evaluated with in vivo 31 C NMR spectroscopy
## Abstract Full signal intensity ^1^Hβ[^13^C] NMR spectroscopy, combining a preceding ^13^Cβediting block based on an __inversion__ BISEP (B~1~βinsensitive spectral editing pulse) with a spinβecho coherenceβbased localization, was developed and implemented at 14.1 T. ^13^C editing of the proposed