1H-[13C] NMR spectroscopy of the rat brain during infusion of [2-13C] acetate at 14.1 T

✍ Scribed by Lijing Xin; Vladimír Mlynárik; Bernard Lanz; Hanne Frenkel; Rolf Gruetter

• Publisher: John Wiley and Sons
• Year: 2010
• Tongue: English
• Weight: 218 KB
• Volume: 64
• Category: Article
• ISSN: 0740-3194
• DOI: 10.1002/mrm.22359

✦ Synopsis

Abstract

Full signal intensity ^1^H‐[^13^C] NMR spectroscopy, combining a preceding ^13^C‐editing block based on an inversion BISEP (B~1~‐insensitive spectral editing pulse) with a spin‐echo coherence–based localization, was developed and implemented at 14.1 T. ^13^C editing of the proposed scheme was achieved by turning on and off the ^13^C adiabatic full passage in the ^13^C‐editing block to prepare inverted and noninverted ^13^C‐coupled ^1^H coherences along the longitudinal axis prior to localization. The novel ^1^H‐[^13^C] NMR approach was applied in vivo under infusion of the glia‐specific substrate [2‐^13^C] acetate. Besides a ∼50% improvement in sensitivity, spectral dispersion was enhanced at 14.1 T, especially for J‐coupled metabolites such as glutamate and glutamine. A more distinct spectral structure at 1.9–2.2 ppm(parts per million) was observed, e.g., glutamate C3 showed a doublet pattern in both simulated ^1^H spectrum and in vivo ^13^C‐edited ^1^H NMR spectra. Besides ^13^C time courses of glutamate C4 and glutamine C4, the time courses of glutamate C3 and glutamine C3 obtained by ^1^H‐[^13^C] NMR spectroscopy were reported for the first time. Such capability should greatly improve the ability to study neuron‐glial metabolism using ^1^H‐observed ^13^C‐edited NMR spectroscopy. Magn Reson Med, 2010. © 2010 Wiley‐Liss, Inc.