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Globin Chain Electrophoresis: a New Approach to the Determination of the Gγ/Aγ Ratio in Fetal Haemoglobin and to Studies of Globin Synthesis

✍ Scribed by Blanche P. Alter; Sabra C. Goff; Georgi D. Efremov; Marsha E. Gravely; Trrus H. J. Huisman

Book ID: 114709378
Publisher: John Wiley and Sons
Year: 1980
Tongue: English
Weight: 829 KB
Volume: 44
Category: Article
ISSN: 0007-1048
DOI: 10.1111/j.1365-2141.1980.tb08706.x

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## Abstract We cultured human adult peripheral and umbilical cord blood mononuclear cells in methylcellulose and measured the synthetic rates of globin chains in individual erythropoietic bursts. Globin chains were labeled with ^14^C‐amino acids in culture, separated by isoelectric focusing in slab