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Glass capillary gas chromatography of amino acid enantiomers

✍ Scribed by Nicholson, Graeme J. ;Frank, Hartmut ;Bayer, Ernst

Publisher
John Wiley and Sons
Year
1979
Tongue
English
Weight
381 KB
Volume
2
Category
Article
ISSN
0935-6304

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✦ Synopsis

Abstract

Glass capillaries coated with Chirasil‐Val, a chirally functionalised polysiloxane, are capable in principle of resolving all protein amino‐acid enantiomers in a single run and within a short analysis time, thus allowing for example the quantitative amino acid determination by enantiomer labelling. The elution characteristics of the individual amino acids however are also dependent upon the chemical nature of the capillary wall surface, and a surface pretreatment is found to be necessary if all protein amino acids are to be analysed. Of the various methods of pretreatment tested, etching of borosilicate glass with gaseous HCl followed by deposition of colloidal silicic acid is considered to be the most suitable.

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