Gibberellin in immature seeds of pharbitis nil

✍ Scribed by Nobutaka Takahashi; Noboru Murofushi; Takao Yokota; Saburo Tamura

• Publisher: Elsevier Science
• Year: 1967
• Tongue: French
• Weight: 205 KB
• Volume: 8
• Category: Article
• ISSN: 0040-4039
• DOI: 10.1016/s0040-4039(00)90637-3

✦ Synopsis

The presence of glbberellln-like substances in immature seeds of morning-glory (Pharbltis a)

has been suggested by Ogawa !

(1,2), Murakami (3) and Zeevaat (4). Now we wish to report the Isolation and structure of a new gibberellln, tentatively named Pharbltis Gibberellln, as well as the presence of gibberellln A3 (GA3) in the seeds. The ethyl acetate-soluble acidic fraction obtained from 60 kg of Immature seeds was successively subjected to ten transfers counter-current distribution method and charcoal chromatography. Elution with acetone-water mixtures, 50:50 and 70:30, gave active fractions, F-I and F-II, respectively. Histogram obtained from thin layer chromatography* and bioassay revealed that different active principles are contained in F-I and F-II. Each fraction was purified through successive procedures: sillcic acid adsorption, partition and preparative thin layer chromatography. F-I thus purified showed a fluorescent spot** at RGA 1.00 on thin layer chromatogram without heating. 3 This spot also exhibited marked growth promoting activity to dwarf maize mutant d-5. When methyl ester of pure F-I was subjected to thin layer chromatography, It revealed a fluorescent spot, whose Rf value was Identical with that of GA 3 methyl ester. Thus, the presence of GA3 In the seeds was established. * Solvent system: for acidic fraction, benzene-n-btitanol-acetic acid (70:25:5, v/v). Adsorbent: Silica gel G. ** When plates are sprayed with 70 $ (v/v) sulfuric acid and heated at 100°, all known gibberellins give blue or green fluorescent spots under ultraviolet light. GA3 and GA7 give fluorescence without heating.