Using in-vivo assays at least two classes of ethylene-binding site are shown to exist in pea epicotyls. These classes appear to have identical affinities for ethylene with a KD of 6 9 10-11-8 9 10 -~1 M; the fast associating class has high and appropriate affinities for physiologically active analog
Gibberellin A20in seed ofPisum sativumL., cv. Alaska
β Scribed by C. J. Eeuwens; P. Gaskin; J. MacMillan
- Book ID
- 104750819
- Publisher
- Springer-Verlag
- Year
- 1973
- Tongue
- English
- Weight
- 176 KB
- Volume
- 115
- Category
- Article
- ISSN
- 0032-0935
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In addition to the previously-reported gibberellins: GA1; GA8, GA20 and GA29 (GarcΓa-MartΓnez et al., 1987, Planta 170, 130-137), GA3 and GA19 were identified by combined gas chromatography-mass spectrometry in pods and ovules of 4-d-old pollinated pea (Pisum sativum cv. Alaska) ovaries. Pods contai
Abscisie acid was eharac~berized from extracts of isolated pea chloroplasts by eombined gas-chromatography-mass spectrometry. By single ion monitoring of the base peak (m/e 190) in the mass spectrum, 8.6 ~xg ABA were deteeted in ehloroplasts isolated from 1 kg fresh weight of pea shoots. \* GC-MS =
Gibberellins A1, As, A2o and A29 were identified by capillary gas chromatography-mass spectrometry in the pods and seeds from 5-d-old pollinated ovaries of pea (Pisum sativum cv. Alaska). These gibberellins were also identified in 4-d-old non-developing, parthenocarpic and pollinated ovaries. The le
Gibberellin A9, GA20 and GA29, all known to be endogenous to immature seeds of Pisum sativum cv. Progress No. 9, were fed in tritiated form to seeds of this cultivar cultured in vitro. [(3)H]GA9 was metabolised to [(3)H]GA20, 2Ξ²-OH-[(3)H]GA9, H2-[(3)H]GA31 and a H2-[(3)H]GA31 conjugate. The percenta