Seven day old seedlings of Pisum sativum L., cv. Kleine RheinlΓ€nderin, were wilted for 3 days. After partially removing the roots, they were rewatered and at the same time radioactive abscisic acid([1-(14)C]ABA, spec. activity 1.7Β·10(8)d s(-1)mmol(-1)) was applied for 1 h via the xylem of the roots.
Characterization of abscisic acid in chloroplasts ofPisum sativumL. cv. Alaska by combined gas-chromatography-mass spectrometry
β Scribed by I. D. Railton; D. M. Reid; P. Gaskin; J. MacMillan
- Publisher
- Springer-Verlag
- Year
- 1974
- Tongue
- English
- Weight
- 228 KB
- Volume
- 117
- Category
- Article
- ISSN
- 0032-0935
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β¦ Synopsis
Abscisie acid was eharac~berized from extracts of isolated pea chloroplasts by eombined gas-chromatography-mass spectrometry. By single ion monitoring of the base peak (m/e 190) in the mass spectrum, 8.6 ~xg ABA were deteeted in ehloroplasts isolated from 1 kg fresh weight of pea shoots. * GC-MS = combined gas chromatography-mass spectrometry.
π SIMILAR VOLUMES
Indole-3-acetic acid (IAA) and abscisic acid (ABA) were identified by combined gas chromatography-mass spectrometry (GCMS) in fractions obtained by diffusion and extraction from bark peelings of Sitka spruce. A procedure is described for the quantitative analysis of IAA and ABA levels in the same ex