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Gentisate dioxygenase activity in immobilized cell-free extracts prepared fromSalmonella typhimurium

✍ Scribed by Frederick E. Goetz; Jeonghui Joo

Book ID: 104650383
Publisher: Springer-Verlag
Year: 1990
Tongue: English
Weight: 260 KB
Volume: 4
Category: Article
ISSN: 0951-208X
DOI: 10.1007/bf00163283

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✦ Synopsis

Cell-free extracts of Salmonella typhimurium containing gentisate dioxygenase were immobilized by entrapment in carrageenan and polyacrylamide and adsorption to polyester filters. Carrageenan gels were very porous, and the enzyme was slowly inactivated in polyacrylamide. When adsorbed on polyester the enzyme could be lyophilized and reactivated. Batch reactors charged with the immobilized enzyme converted gentisate to maleylpyruvate with yields of 10-90%.

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