Preparation of a cell-free extract from yeast that is active in protein synthesis
โ Scribed by Christian Kreutzfeldt; Ernst-Randolf Lochmann
- Book ID
- 109312156
- Publisher
- John Wiley and Sons
- Year
- 1983
- Tongue
- English
- Weight
- 287 KB
- Volume
- 16
- Category
- Article
- ISSN
- 0378-1097
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
Wheat embryo extracts are commonly used in cell-free protein synthesis studies. Soluble inhibitor(s) that diminish aminoacylation of tRNA exist in such extracts. These inhibitors can be effectively removed by prolonged dialysis, provided that proper salt conditions are maintained to ensure stability
Ricinus communis agglutinin II (RCAII, ricin, toxin) at low concentrations inhibits protein synthesis in cell-free extracts, but not in intact cells, of an RCAII-resistant mouse lymphoma variant cell line. The concentration dependence of the inhibition by RCAII was the same in cell-free extracts of