Genotyping: Methods and Protocols

✍ Scribed by Stefan J. White, Stuart Cantsilieris (eds.)

Publisher: Humana Press
Year: 2017
Tongue: English
Leaves: 256
Series: Methods in Molecular Biology 1492
Edition: 1
Category: Library

No coin nor oath required. For personal study only.

✦ Synopsis

This volume explores a broad range of different genotyping techniques. Genotyping: Methods and Protocols consists of chapters that cover numerous topics such as: an overview of multiplexed microsatellite analysis; High Resolution Melt analysis and TaqMan-based assays; in situ analysis of variants in single RNA molecules; the MassARRAY system and Molecular Inversion Probes; Pulsed Field Gel Electrophoresis, Paralogue Ratio Test, and Multiplex Ligation-Dependent Probe Amplification; long-range PCR combined with PacBio sequencing; Targeted Locus Amplification; Multilocus Sequence Typing and rapid SNP detection with pyrosequencing; and genotyping-by-sequencing for plant analysis. Finally, the volume concludes with a summary of pertinent points to describe genetic variation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Thorough and practical, Genotyping: Methods and Protocols is a valuable resource for anyone interested in learning more about the diverse field of genotyping.

✦ Table of Contents

Front Matter....Pages i-x
Back Matter....Pages 1-15
....Pages 17-28

✦ Subjects

Human Genetics

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