Genotype Phenotype Coupling. Methods and Protocols

✍ Scribed by Stefan Zielonka (ed.), Simon Krah (ed.)

• Publisher: Humana Press
• Year: 2020
• Tongue: English
• Leaves: 447
• Series: Methods in Molecular Biology 2070
• Category: Library

✦ Synopsis

This volume aims at providing state-of-the-art protocols detailing ribosome display, cDNA display, phage display, yeast surface display, and mammalian display. Chapters guide readers through methods and protocols on in vitro methods over prokaryotic display systems, lower eukaryotes, and mammalian cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of all necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Genotype Phenotype Coupling aims to provide an overview of current technologies in this exciting and continuously evolving field.

✦ Table of Contents

Front Matter ....Pages i-xii
Screening, Post-SELEX Optimization and Application of DNA Aptamers Specific for Tobramycin (Nandi Zhou, Rongfeng Cai, Xuyan Han)....Pages 1-18
Affitins: Ribosome Display for Selection of Aho7c-Based Affinity Proteins (Valentina Kalichuk, Stanimir Kambarev, Ghislaine Béhar, Benjamin Chalopin, Axelle Renodon-Cornière, Barbara Mouratou et al.)....Pages 19-41
cDNA Display: A Stable and Simple Genotype–Phenotype Coupling Using a Cell-Free Translation System (Hidenao Arai, Shigefumi Kumachi, Naoto Nemoto)....Pages 43-56
cDNA Display of Disulfide-Containing Peptide Library and In Vitro Evolution (Tai Kubo, Mohammed Naimuddin)....Pages 57-77
Rapid Antigen and Antibody-Like Molecule Discovery by Staphylococcal Surface Display (Marco Cavallari)....Pages 79-94
Restriction-Free Construction of a Phage-Presented Very Short Macrocyclic Peptide Library (Valentin Jakob, Saskia Helmsing, Michael Hust, Martin Empting)....Pages 95-113
In Vitro Maturation of a Humanized Shark VNAR Domain to Improve Its Biophysical Properties (John Steven, Obinna C. Ubah, Magdalena Buschhaus, Marina Kovaleva, Laura Ferguson, Andrew J. Porter et al.)....Pages 115-142
Antibody Phage Display: Antibody Selection in Solution Using Biotinylated Antigens (Esther V. Wenzel, Kristian D. R. Roth, Giulio Russo, Viola Fühner, Saskia Helmsing, André Frenzel et al.)....Pages 143-155
Assessing Antibody Specificity in Human Serum Using Deep Sequence-Coupled Biopanning (Kathryn M. Frietze, Susan B. Core, Alexandria Linville, Bryce Chackerian, David S. Peabody)....Pages 157-171
Isolation of Antigen-Specific VHH Single-Domain Antibodies by Combining Animal Immunization with Yeast Surface Display (Lukas Roth, Simon Krah, Janina Klemm, Ralf Günther, Lars Toleikis, Michael Busch et al.)....Pages 173-189
Selection and Characterization of Anti-idiotypic Shark Antibody Domains (Doreen Könning, Stefan Zielonka, Anna Kaempffe, Sebastian Jäger, Harald Kolmar, Christian Schröter)....Pages 191-209
Simplifying the Detection of Surface Presentation Levels in Yeast Surface Display by Intracellular tGFP Expression (Steffen C. Hinz, Adrian Elter, Julius Grzeschik, Jan Habermann, Bastian Becker, Harald Kolmar)....Pages 211-222
Methods for Construction of Yeast Display Libraries of Four-Domain T-Cell Receptors (Flávio Sádio, Gerhard Stadlmayr, Katja Eibensteiner, Katharina Stadlbauer, Florian Rüker, Gordana Wozniak-Knopp)....Pages 223-248
Isolation of Tailor-Made Antibody Fragments from Yeast-Displayed B-Cell Receptor Repertoires by Multiparameter Fluorescence-Activated Cell Sorting (Anna Kaempffe, Sebastian Jäger, Doreen Könning, Harald Kolmar, Christian Schröter)....Pages 249-266
Isolation of Anti-Hapten Antibodies by Fluorescence-Activated Cell Sorting of Yeast-Displayed B-Cell Receptor Gene Repertoires (Sebastian Jäger, Simon Krah, Doreen Könning, Janis Rosskopf, Stephan Dickgiesser, Nicolas Rasche et al.)....Pages 267-287
Rapid Generation of Chicken Immune Libraries for Yeast Surface Display (Jan P. Bogen, Julius Grzeschik, Simon Krah, Stefan Zielonka, Harald Kolmar)....Pages 289-302
Ligand Engineering via Yeast Surface Display and Adherent Cell Panning (Lawrence A. Stern, Patrick S. Lown, Benjamin J. Hackel)....Pages 303-320
Simultaneous Soluble Secretion and Surface Display of Proteins in Saccharomyces cerevisiae Using Inefficient Ribosomal Skipping (Carlos A. Cruz-Teran, Kaitlyn Bacon, Balaji M. Rao)....Pages 321-334
Chemical Modification of the Yeast Cell Surface Allows the Switch Between Display and Soluble Secretion of Full-Length Antibodies (Simon Krah, Ralf Günther, Stefan Becker, Stefan Zielonka, Laura Rhiel)....Pages 335-349
Advanced Establishment of Stable Recombinant Human Suspension Cell Lines Using Genotype–Phenotype Coupling Transposon Vectors (Karen Berg, Vanessa Nicole Schäfer, Natalie Tschorn, Jörn Stitz)....Pages 351-361
Mammalian Surface Display Screening of Diverse Cystine-Dense Peptide Libraries for Difficult-to-Drug Targets (Zachary R. Crook, Gregory P. Sevilla, Andrew J. Mhyre, James M. Olson)....Pages 363-396
Engineering Antibodies on the Surface of CHO Cells (Annalee W. Nguyen, Kevin Le, Jennifer A. Maynard)....Pages 397-422
Single B Cell Cloning and Production of Rabbit Monoclonal Antibodies (Juliet Rashidian, Joshua Lloyd)....Pages 423-441
Back Matter ....Pages 443-445