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Genotypic differences in callus induction and plant regeneration from mature embryos of barley (Hordeum vulgareL.)

✍ Scribed by Yong Han; Xiao-li Jin; Fei-bo Wu; Guo-ping Zhang

Book ID
111842922
Publisher
SP Zhejiang University Press
Year
2011
Tongue
English
Weight
584 KB
Volume
12
Category
Article
ISSN
1673-1581

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Callus was induced in standard (1 mm) leaf base explants and in cross sections (microexplants) through the seedling axis from seedlings of Hordeum vulgare L.. Reduced callus formation was observed with increasing distance from the leaf base, and explants from the first and second leaves gave the bes

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Plant regeneration from calli was carried out at two locations using several parental genotypes and environments. Selfed immature diploid (VV) barley (Hordeum vulgare) embryos and immature haploid (V) or hybrid (VB) embryos from crosses between H. vulgare and H. bulbosum were used as explants. 'Gold