Genotoxicity Assessment: Methods and Protocols

✍ Scribed by Alok Dhawan, Mahima Bajpayee

• Publisher: Springer New York;Humana
• Year: 2019
• Tongue: English
• Leaves: 376
• Series: Methods in Molecular Biology 2031
• Edition: 2nd ed. 2019
• Category: Library

✦ Synopsis

This current edition explores new tests for genotoxicity testing, along with other well-known techniques. This will further help in our understanding of the genotoxic effects of chemicals. The book has different sections dealing with various assays for gene mutation, chromosomal abnormalities, primary DNA damage, etc. It also delves into plant models, animals and their alternates, as well as in silico approaches for genetic toxicology. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, tips on troubleshooting and avoiding known pitfalls.
Authoritative and up-to-date, Genotoxicity Assessment: Methods and Protocols, Second Edition serves as a highly useful and ready resource for research students and scientists working in regulatory toxicology as well as biomedical, biochemical, and pharmaceutical sciences.

✦ Table of Contents

Front Matter ....Pages i-xi
Front Matter ....Pages 1-1
In Vitro Mouse Lymphoma Cell (L5178Y Tk+/− −3.7.2.C) Forward Mutation Assay (Melissa R. Schisler, Martha M. Moore, B. Bhaskar Gollapudi)....Pages 3-28
Erythrocyte-Based Pig-a Gene Mutation Assay (Jeffrey C. Bemis, Nikki E. Hall, Stephen D. Dertinger)....Pages 29-57
Detection of In Vivo Mutation in the Hprt and Pig-a Genes of Rat Lymphocytes (Vasily N. Dobrovolsky, Joseph G. Shaddock, Roberta A. Mittelstaedt, Daishiro Miura, Robert H. Heflich)....Pages 59-75
Front Matter ....Pages 77-77
In Vitro Cytogenetic Assays: Chromosomal Aberrations and Micronucleus Tests (Pasquale Mosesso, Serena Cinelli)....Pages 79-104
Dicentric Chromosome Assay (DCA) and Cytokinesis-Block Micronucleus (CBMN) Assay in the Field of Biological Dosimetry (Antonella Testa, Valentina Palma, Clarice Patrono)....Pages 105-119
Chromosomal Aberration Test in Human Lymphocytes (Christian Johannes, Guenter Obe)....Pages 121-134
In Vivo Micronucleus Assay in Mouse Bone Marrow (Abhishek Kumar Jain, Alok Kumar Pandey)....Pages 135-146
Micronucleus Cytome Assays in Human Lymphocytes and Buccal Cells (Claudia Bolognesi, Michael Fenech)....Pages 147-163
Micronucleus Analysis by Flow Cytometry (Azeddine Elhajouji, Pascal Stadelmann)....Pages 165-193
Hen’s Egg Test for Micronucleus Induction (HET-MN) (Kerstin Reisinger, Eva Dony, Thorsten Wolf, Katrin Maul)....Pages 195-208
Comparative Genomic Hybridization (CGH) in Genotoxicology (Adi Baumgartner, Veronika Hartleb, Jim D. Taylor)....Pages 209-234
Front Matter ....Pages 235-235
The Comet Assay: Assessment of In Vitro and In Vivo DNA Damage (Mahima Bajpayee, Ashutosh Kumar, Alok Dhawan)....Pages 237-257
The Comet Assay in Human Biomonitoring (Diana Anderson, Alok Dhawan, Julian Laubenthal)....Pages 259-274
The Comet Assay in Marine Animals (Patrizia Guidi, Brett P. Lyons, Giada Frenzilli)....Pages 275-286
Multicolor Laser Scanning Confocal Immunofluorescence Microscopy of DNA Damage Response Biomarkers (Julian Laubenthal, Michal R. Gdula, Alok Dhawan, Diana Anderson)....Pages 287-300
Detection of DNA Double-Strand Breaks Using Pulsed-Field Gel Electrophoresis (Vyom Sharma, Vishwa Mohan)....Pages 301-311
Detection of UV-Induced Thymine Dimers (Vipin Kumar Yadav, Poorwa Awasthi, Amit Kumar)....Pages 313-322
Front Matter ....Pages 323-323
Micronucleus Assay with Tetrad Cells of Tradescantia (Miroslav Mišík, Clemens Pichler, Bernhard Rainer, Armen Nersesyan, Katarína Mišíková, Siegfried Knasmueller)....Pages 325-335
The Wing-Spot and the Comet Tests as Useful Assays for Detecting Genotoxicity in Drosophila (Ricard Marcos, Erico R. Carmona)....Pages 337-348
Front Matter ....Pages 349-349
In Silico Approaches in Predictive Genetic Toxicology (Meetali Sinha, Alok Dhawan, Ramakrishnan Parthasarathi)....Pages 351-373
Back Matter ....Pages 375-378

✦ Subjects

Biomedicine; Pharmacology/Toxicology; Human Genetics; Ecotoxicology