Genome sequence of the recombinant protein production host Pichia pastoris
✍ Scribed by De Schutter, Kristof; Lin, Yao-Cheng; Tiels, Petra; Van Hecke, Annelies; Glinka, Sascha; Weber-Lehmann, Jacqueline; Rouzé, Pierre; Van de Peer, Yves; Callewaert, Nico
- Book ID
- 109900673
- Publisher
- Nature Publishing Group
- Year
- 2009
- Tongue
- English
- Weight
- 637 KB
- Volume
- 27
- Category
- Article
- ISSN
- 1087-0156
- DOI
- 10.1038/nbt.1544
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The Pichia pastoris expression system offers economy, ease of manipulation, the ability to perform complex post-translational modifications, and high expression levels. Using this system, recent advances have been made in the quality of recombinant proteins in fermenter culture and in the quality of
## Abstract A method combining fluorescence activated cell sorting (FACS) and DNA microarray assisted clone identification was developed and termed Genome‐Scale Analysis of Library Sorting (GALibSo). Genes enhancing the production of secreted heterologous proteins in __Pichia pastoris__ were identi