𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Genome resource for the indonesian coelacanth,Latimeria menadoensis

✍ Scribed by Danke, Joshua ;Miyake, Tsutomu ;Powers, Thomas ;Schein, Jacqueline ;Shin, Heesun ;Bosdet, Ian ;Erdmann, Mark ;Caldwell, Roy ;Amemiya, Chris T.

Book ID
102337509
Publisher
John Wiley and Sons
Year
2004
Tongue
English
Weight
206 KB
Volume
301A
Category
Article
ISSN
0022-104X

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

We have generated a BAC library from the Indonesian coelacanth, Latimeria menadoensis. This library was generated using genomic DNA of nuclei isolated from heart tissue, and has an average insert size of 171 kb. There are a total of 288 384‐well microtiter dishes in the library (110,592 clones) and its genomic representation is estimated to encompass β‰₯7X coverage based on the amount of DNA presumably cloned in the library as well as via hybridization with probes to a small set of single copy genes. This genomic resource has been made available to the public and should prove useful to the scientific community for many applications, including comparative genomics, molecular evolution and conservation genetics. J. Exp. Zool. 301A:228–234, 2004. Β© 2004 Wiley‐Liss, Inc.

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Medical Research Council (MRC) funded UK Human Genome Mapping Project Resource Centre (HGMP-RC), on the Wellcome Trust Genome Campus at Hinxton, near Cambridge, UK. The Centre provides both biological and bioinformatics resources to the UK genomics community, and also has research groups supporting